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Fig. 4
MMP activity is increased in ML-II embryos. (A–C) General MMP activity was measured in WT and ML-II embryo lysates across a developmental time course (1–4 dpf; A), following introduction of GNPTAB mRNA (3 dpf; B) and after incubation with EDTA (inhibits MMPs) or APMA (activates MMPs) for 2 hours (3 dpf; C). (D) Activity of MMP-12 and/or -13 in 3-dpf WT and ML-II embryos measured using specific fluorogenic substrate. (E) MMP-13 immunoblots show increased steady-state levels of both the MMP-13 precursor (48 kDa) and the mature active (35 kDa) forms in 3-dpf ML-II embryo lysates. β-tubulin was used as a loading control. (F) General MMP activity assays in isolated feline synovial fibroblast-like cell lysates. *P<0.05, **P<0.01, ***P<0.001, Student’s t-test. Activity measurements for all conditions were performed on at least three independent embryo lysates.