FIGURE

Fig. 8

ID

ZDB-FIG-111128-78

Publication

Muto et al., 2011 - Multifactorial Origins of Heart and Gut Defects in nipbl-Deficient Zebrafish, a Model of Cornelia de Lange Syndrome

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Fig. 8

Distinct effects of Nipbl and cohesin on gene expression.

(A) Expression of sox32 (upper) and sox17 (lower) in uninjected (left column), nipbla/b-morphants (middle column) and smc3-morphants (right column) was examined by in situ hybridization at 8.3 hpf (80% epiboly). Views are dorsal with anterior at top. (B) Gene expression changes in nipbla/b- and cohesin-morphants. Gene expression was examined in embryos injected with nipbla/b-MO1, smc3-MO, and rad21-MO by Q-PCR at 6.5 hpf or 26 hpf (as indicated). Expression levels are presented relative to those in uninjected embryos (100%; n = 3, * p<0.01, ** p<0.05). (C) Levels of Smc3, Nipbla, and Nibplb protein were examined by Western blotting at 9 hpf. α-Tubulin was used as a loading control. Amounts of MO injected in each embryo were: nipbla/b-MO1, 0.75 ng each; smc3-MO, 4 ng; rad21-MO, 5 ng. (D) Gross morphologies of cohesin-morphants at 25 hpf. Views are lateral with anterior to the left.

Expression Data

Genes:	foxa2 sox17 sox32
Fish:	AB AB + MO1-smc3 AB + MO2-rad21a AB + MO1-nipbla + MO1-nipblb
Knockdown Reagents:	MO1-nipbla MO1-nipblb MO1-smc3 MO2-rad21a
Anatomical Terms:	endoderm whole organism
Stage Range:	Shield to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-smc3 AB + MO2-rad21a
Knockdown Reagents:	MO1-smc3 MO2-rad21a
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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