FIGURE

Fig. 1

ID

ZDB-FIG-111128-28

Publication

Wang et al., 2011 - Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Negative and positive regulation of sox6 in the zebrafish myotome. (A,B) sox6 expression is restricted to the fast muscle domain in a wild-type 10-somite stage embryo (A) but is ectopically expressed in adaxial cells in a prdm1a^nrd mutant at the same stage. (C,D) This derepression correlates with downregulation of stnnc expression in adaxial cells in the prdm1a^nrd mutant (D) relative to wild type (C). (E,F) sox6 transcript persists in the fast-twitch muscle domain and also accumulates in the otic placode in the wild type at the 18-somite stage (E), whereas the myod1;myf5 morphant shows loss of sox6 expression specifically from the muscle progenitors. (G,H) Expression of myod1 in wild-type (G) and myod1;myf5 morphant (H) embryos. Scale bars: 50 μm.

Expression Data

Genes:	myod1 sox6 tnnc1b
Fish:	WT prdm1a^m805/m805 WT + MO1-myf5 + MO1-myod1
Knockdown Reagents:	MO1-myf5 MO1-myod1
Anatomical Term:	somite fast muscle cell
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development