FIGURE

Fig. 3

ID

ZDB-FIG-110928-5

Publication

Liu et al., 2011 - Genetic Association for Renal Traits among Participants of African Ancestry Reveals New Loci for Renal Function

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Fig. 3

Kcnq1 knockdown in zebrafish embryos causes glomerular abnormalities.

(A–E) Zebrafish embryos injected with control morpholino show normal glomerular and tubular morphology, as shown by in situ hybridization for the global kidney marker pax2a (A, inset showing lower-magnification image, with staining in both glomerulus and tubules), the podocyte markers wt1a (B) and nephrin (C), and the proximal and distal tubular markers slc20a1a (D) and slc12a3 (E). (F–J) Injection of the kcnq1 antisense morpholino, targeting the ATG site of the gene, at the one-cell stage results in significant changes in glomerular gene expression (F–H). No changes were observed in the proximal tubule (I) or the distal tubule (J).

Expression Data

Genes:	nphs1 pax2a slc12a3 slc20a1a wt1a
Fish:	WT WT + MO1-kcnq1.1
Knockdown Reagent:	MO1-kcnq1.1
Anatomical Terms:	podocyte pronephric distal late tubule pronephric glomerulus pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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