FIGURE

Fig. 5

ID

ZDB-FIG-110712-5

Publication

Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin

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Fig. 5

Inhibiting Nodal signals in MZmid mutants.

Wild-type (A), uninjected MZmid (B), and injected MZmid (C–E) embryos compared to a complete loss of Nodal signaling in MZoep (F) mutants. Inhibiting bon function (3 ng bonMO) in an MZmid background further impairs anterior development but does not affect tail development (C). Blocking eomes function (15–20 pg eomes-enR mRNA; D) resembles both a loss of the Nodal ligands cyc and sqt (8 ng each cycMO+sqtMO; E) and MZoep (F), indicating that Nodal signaling is occurring in MZmid mutants and is mediated by Eomes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxh1^pr1/pr1 tdgf1^tz257/tz257 foxh1^pr1/pr1 + MO1-mixl1 foxh1^pr1/pr1 + MO1-ndr2 + MO4-ndr1
Knockdown Reagents:	MO1-mixl1 MO1-ndr2 MO4-ndr1
Observed In:	brain dorsal/ventral pattern formation extension eye head neural plate anterior/posterior regionalization notochord pericardium post-vent region somite whole organism anterior-posterior axis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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