FIGURE

Fig. 3

ID

ZDB-FIG-110712-3

Publication

Slagle et al., 2011 - Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin

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Fig. 3

sur retains more Nodal transduction capability than mid.

(A) Genetic interactions between the sur and mid alleles, demonstrating the ability of sur to partially rescue the loss of notochord caused by mid. Lower panels are enlargements showing the structures of the notochords at 24 hpf; red brackets indicate notochord domains. (B) Nodal overexpression in maternal-zygotic FoxH1 mutants. 50 pg sqt RNA was injected into wild-type, MZsur, and MZmid embryos at the one-cell stage, and embryos were assayed for Nodal target gene expression at 30–40% epiboly. Note the greater ability of MZsur embryos to respond to ectopic sqt compared to the MZmid response. (C) The mid mutation perturbs activin-like signaling. MZoep and MZmid embryos were injected with RNA encoding either a Xenopus activin homologue (2.5 pg) or an activated form of mouse Smad2 (100 pg). Responses were assayed by observing ntl expression at 30–40% epiboly.

Expression Data

Genes:	gsc tbxta
Fish:	AB foxh1^m768/m768 foxh1^pr1/pr1 tdgf1^tz257/tz257
Anatomical Terms:	margin presumptive shield
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxh1^m768/m768 foxh1^pr1/pr1 foxh1^m768/+; foxh1^pr1/+
Observed In:	notochord somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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