FIGURE

Fig. 3

ID

ZDB-FIG-110111-36

Publication

Bresciani et al., 2010 - Zebrafish numb and numblike are involved in primitive erythrocyte differentiation

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Fig. 3

Expression of hematopoietic genes in nb/nbl morphants.

A–D. Flat mounted 10-ss embryos. Triple WISH were performed on std MO (A, C) and nb/nbl MO-injected embryos (B, D). The expression of myoD in somitic and pre-somitic mesoderm appeared unaltered in nb/nbl MO embryos (B, D). The expression of krox20 in the rombomeres 3 and 5 is unaffected in nb/nbl morphants (B, D). A–B. The expression of scl appears reduced in the posterior PLM (black arrowheads). C–D. gata1 expression is reduced in nb/nbl morphants, particularly in the posterior PLM (black arrowheads). E–N. Lateral view of 22–24 hpf std MO embryos (E, G, I, M) and nb/nbl morphants (F, H, L, N). In nb/nbl morphants at 22 hpf gata1 expression is reduced in the ICM (F); ikaros and βe1 globin are also downregulated at 24 hpf (H, L). The myeloid marker pu.1 is expressed at normal levels in nb/nbl MO-injected embryos (N).

Expression Data

Genes:	egr2b gata1a hbbe1.1 ikzf1 myod1 spi1b tal1
Fish:	WT WT + MO2-numb,numbl
Knockdown Reagent:	MO2-numb,numbl
Anatomical Terms:	erythroid lineage cell hematopoietic multipotent progenitor cell intermediate cell mass of mesoderm myeloid cell posterior lateral plate mesoderm rhombomere 3 rhombomere 5 segmental plate somite
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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