Fig. 4

han^c99 Mutants Exhibit Prolonged Deposition of Laminin at the LPM/Gut Boundary

(A–F) Time course analysis of Tg(hand2:EGFP) expression and laminin deposition during LPM migration. Ten wild-type (A–C) and eight han^c99 mutants (D–F) were fixed at the stages indicated and stained for laminin (red) and GFP (green). Dashed lines outline the notochord.

(A–C) In wild-type, laminin becomes diminished along the migratory path of Tg(hand2:EGFP)-expressing cells (arrows).

(D–F) In han^c99 mutants, laminin deposition persists along the entire LPM/gut boundary (arrows).

(G) The LPM in lamβ1^s804 heterozygotes undergoes asymmetric migration (28/30).

(H–J) Out of 29 lamβ1^s804 mutants examined, 6 showed incomplete asymmetric LPM migration (H), 18 had both the left and right LPM remain on top of the gut (I), and 5 had the LPM located lateral to the gut (J). Arrows point to the aberrant intrusion of LPM cells into the gut. Dashed lines outline the LPM.

(K) Percentages (mean ± SEM) of han^c99 mutants (n = 21) from han^c99+/- incrosses and han^c99 single mutants (n = 56) from han^c99+/-;lamβ1^s804+/- incrosses showing gut-looping phenotypes according to foxa3 expression. Three independent crosses were examined.

All images are transverse sections, dorsal to the top. L, left; NC, notochord; R, right. The scale bars represent 40 μm. See also Figure S3.