FIGURE

Fig. 8

ID

ZDB-FIG-101122-37

Publication

Nowak et al., 2005 - Perp is required for tissue-specific cell survival during zebrafish development

Other Figures

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Fig. 8

Loss of Perp causes caspase-dependent apoptosis. Acridine orange (a–f) and FITC-VAD-fmk (h,i) stainings of live embryos at 30 hpf; (a,c,e,h) injected with perp MO, (b) injected with perp MO and treated with caspase inhibitor zVAD-fmk, (c,f,i) injected with perp 4 mm MO, (d) uninjected control; (a–d,h,i) lateral view on tip of tail; (e,f) magnified lateral view on notochord. Note that in the notochord of perp morphants (e), all cells display altered, roundish shapes, while only some of the cells have become acridine orange positive. (g) Diagram illustrating average numbers of acridine orange-positive cells in embryos as shown in (a–d) within the tail region posterior of the anus. Number of evaluated embryos: control n=5; perp 4 mm MO-injected n=5; perp MO-injected n=6; perp 4 mm MO-injected+zVAD-FMK n=8

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-perp
Knockdown Reagent:	MO1-perp
Observed In:	integument integument epithelial cell notochord ventral fin fold cell
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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ZFIN also wishes to thank Matthias Hammerschmidt for permission to reproduce figures from this article.

Full text @ Cell Death Differ.