FIGURE

Fig. 2

ID

ZDB-FIG-101119-21

Publication

Cao et al., 2009 - Chemical modifier screen identifies HDAC inhibitors as suppressors of PKD models

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Inhibition of Class I HDACs can suppress the phenotypes of pkd2 mutants/morphants. (A and B) Treatment of 20 μm VPA from the shield stage leads to slight ventral curvature (B) on 2 dpf in wild-type embryos (wt) as compared to embryos treated with DMSO (A). (C–E) VPA can suppress the body curvature of pkd2/hi4166 mutant embryos. (C) shows mutant embryos treated with DMSO on 2 dpf. (D) shows mutant embryos treated with 20 μm VPA from 27 hpf on 2 dpf. (E) Average curvature angle in embryos treated with 20 μm VPM and 20 &,mu;m VPA. n = 5, *, P < 0.005, (F–H) Inhibition of kidney cyst formation in pkd2 morphants (pkd2mo) on 3 dpf by treatment of 20 μm VPA from the 20-somite stage. (F) shows a pkd2 morphants treated with 20 μm VPM. The arrow points to the cyst. (G) shows a morphant treated with VPA. White box: area shown in Insets. Insets show kidney cyst in F or the lack of cyst in G. (H) Average of percentage of embryos with kidney cysts from three independent experiments. Twenty-five to one hundred embryos were examined in each experiment. *, P < 0.005. 4166: embryos from pkd2/hi4166 heterozygous cross.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU pkd2^{hi4166Tg/hi4166Tg} (AB/TU) AB/TU + MO3-pkd2
Conditions:	chemical treatment by environment: valproic acid chemical treatment by environment: valpromide
Knockdown Reagent:	MO3-pkd2
Observed In:	pronephros whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA