FIGURE

Fig. S6

ID

ZDB-FIG-100809-35

Publication

Xie et al., 2010 - A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development

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Fig. S6

Morpholino knockdown of claudin 5a in zebrafish embryos results in BRB breakdown. Cldn-5a was knocked down by injecting morpholino-cldn5a into double transgenic l-fabp:DBP-EGFP;flk1:mCherry embryos at the 2-cell stage. At 4 dpf, leakage of DBP-EGFP(green) from the hyaloid vasculature (red) was observed. A 5 bp-mismatched Morpholino was injected as control and showed an intact BRB.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lri500Tg; s896Tg + MO2-cldn5a
Knockdown Reagent:	MO2-cldn5a
Observed In:	establishment of endothelial barrier hyaloid vessel
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.