FIGURE

Fig. S7

ID

ZDB-FIG-100225-17

Publication

Sun et al., 2010 - Zili Inhibits TGF-beta Signaling by Interacting with Smad4

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Fig. S7

Validation of interaction between Zili and Smad4 by Two-Hybrid experiment in Bacterial system. The BacterioMatch II two-hybrid system detects protein-protein interactions based on transcriptional activation of the HIS3 reporter gene, which allows growth in the presence of 3-amino-1,2,4-triazole (3-AT), a competitive inhibitor of His3 enzyme. Both pBT-Zili and pTRG-Smad4 can not produce a significant number of colonies on Selective Screening Medium (5 mM 3-AT) alone, suggesting that they are suitable for use in this system. And significant growth on 5 mM 3-AT is observed in cotransformation experiment of pBT-Zili and pTRG-Smad4, similar to the pTRG-Gal11P and the pBT-LGF2 positive control plasmids, indicating an interaction.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ J. Biol. Chem.