FIGURE

Fig. 2

ID

ZDB-FIG-100128-18

Publication

Mitchell et al., 2010 - Effect of Vascular Cadherin Knockdown on Zebrafish Vasculature during Development

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Fig. 2

VE-Cad MO splice-blockade also impairs formation of circulation and results in pericardial edema and improper cardiac looping.

Brightfield images of embryos injected with VE-Cad splice-blocking morpholino (VE-Cad spMO) also show impaired circulation, pericardial edema and impaired cardiac looping (c–d, g–h), when compared to controls (a–b, e–f). Assessments of cardiovascular phenotype: (i) circulation was assessed in VE-cad tMO, spMO and control MO embryos, with VE-cad MO injected embryos demonstrating impaired circulation at all time points. (j) Pericardial edema was highly prevalent in VE-cad MO injected embryos. (k) Cardiac looping in the two groups, with abnormal looping more frequent in the VE-cad MO fish (n = 150–200 embryos per group).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cdh5 AB + MO5-cdh5
Knockdown Reagents:	MO1-cdh5 MO5-cdh5
Observed In:	blood circulation heart looping pericardium
Stage Range:	Prim-15 to Day 4

Phenotype Detail

Acknowledgments

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