Fig. 3
- ID
- ZDB-FIG-091215-22
- Publication
- Yue et al., 2009 - Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression
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β-Arrestin1 Critically Regulates Zebrafish Hematopoiesis (A–F) Whole-mount double in situ hybridization for embryos at 13 hpf. Embryos injected with 4 ng control morpholino (Ctrl MO) or 4 ng β-arrestin1 morpholino (Arrb1 MO) were hybridized with riboprobes to early hematopoietic precursor marker scl (A and B), erythroid marker gata1 (C and D), and myeloid marker pu.1 (E and F). Embryos were flat-mounted with anterior to the left. Riboprobes to myoD was used to mark the somites for stage matching (red). (G–N) Whole-mount in situ hybridization for embryos at 24 hpf. Embryos injected as (A-H) were hybridized with riboprobes to scl (G and H), hemoglobin marker hbbe3 (I and J), lmo2 (K and L), gata1 (M and N). Embryos are lateral views with anterior to the left. Arrows indicated the position of intermediate cell mass (ICM). (O–R) Embryos derived from transgenic zebrafish lines Tg(lmo2:Rsred) (O and P) and Tg(gata1:EGFP) (Q and R) were injected with β-arrestin1 morpholino and showed significantly decreased levels of fluorescent intensity. Embryos are lateral views with anterior to the left. (S–V) Depletion of β-arrestin1 displayed decreased hemoglobin staining by O-dianisidine at 36 hpf and 72 hpf (arrows). |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | 5-9 somites to Prim-5 |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | Prim-25 to Protruding-mouth |
Reprinted from Cell, 139(3), Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G., Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression, 535-546, Copyright (2009) with permission from Elsevier. Full text @ Cell