FIGURE

Fig. S2

ID

ZDB-FIG-091016-23

Publication

Emond et al., 2009 - Protocadherin-19 is essential for early steps in brain morphogenesis

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Fig. S2

Specificity of anti-Pcdh19 antibody. A, Shown is a Western blot of cell extracts from COS cells that were untransfected or transfected with Pcdh19-GFP or Pcdh19-myc. In untransfected cells, no band is present, while in transfected cells, our antibody detects strong bands corresponding to the sizes of Pcdh19-GFP and Pcdh19-myc, respectively. B–D, Immunocytochemistry of COS cells transfected with zebrafish Pcdh19-GFP. B, Pcdh19-GFP is present in a perinuclear compartment and diffusely along the plasma membrane, as well as in discrete, punctate structures. C, Antibody labeling shows an identical distribution to the Pcdh19-GFP. D, An overlay shows excellent co-localization of Pcdh19-GFP (green) and anti-Pcdh19 (red), while there is no immunolabeling of untransfected cells in the same field (DAPI, blue).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Emond, M.R., Biswas, S., and Jontes, J.D., Protocadherin-19 is essential for early steps in brain morphogenesis, 72-83, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.