Fig. 8

Disruption of pcdh19 function does not alter axial patterning or somitogenesis. A–C, To assess the effects of pcdh19 depletion on regional specification in the brain, we performed in situ hybridizations on 10 somite stage embryos using riboprobes for pax2.1, which labels the midbrain–hindbrain boundary, and krox20, which labels rhombomeres 3 and 5. Compared to control embryos (A), the morphants (B, Mo-S1; C, Mo-T) exhibited comparable patterns of pax2.1 and krox20 expression, although the neural plates are substantially wider (compare B′ and C′ with A′). D–F, Similar to what we observe with pax2.1/krox20, the midbrain region, marked by otx2, appears normal in morphant embryos. G–I, Transmitted light images of control (G, Mo-S1mis), and pcdh19 morphant embryos (H, Mo-S1; I, Mo-T). While convergence of the anterior neural plate appears to be impaired in pcdh19 morphants, convergence in the trunk is unaffected. J–L, To confirm that morphogenesis in the trunk is normal, 8–9 somite stage embryos were labeled with riboprobe against myod.