Fig. S2

Fig. S2 Specificity of anti-Pcdh19 antibody. A, Shown is a Western blot of cell extracts from COS cells that were untransfected or transfected with Pcdh19-GFP or Pcdh19-myc. In untransfected cells, no band is present, while in transfected cells, our antibody detects strong bands corresponding to the sizes of Pcdh19-GFP and Pcdh19-myc, respectively. B–D, Immunocytochemistry of COS cells transfected with zebrafish Pcdh19-GFP. B, Pcdh19-GFP is present in a perinuclear compartment and diffusely along the plasma membrane, as well as in discrete, punctate structures. C, Antibody labeling shows an identical distribution to the Pcdh19-GFP. D, An overlay shows excellent co-localization of Pcdh19-GFP (green) and anti-Pcdh19 (red), while there is no immunolabeling of untransfected cells in the same field (DAPI, blue).