FIGURE

Fig. S2

ID

ZDB-FIG-090831-45

Publication

French et al., 2009 - Gdf6a is required for the initiation of dorsal-ventral retinal patterning and lens development

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Fig. S2

Morpholino inhibition of bmp4 has no effect on eye patterning. Phosphorylation of Smad 1/5/8 proteins is not affected at any stage tested, including 6 somites (A, D), 10 somites (B, F), or 28 hpf (C, G). Similarly, the expression of tbx5 is normal at 6 somites (H, K) 10 somites (I, L) and 28 hpf (J, M). Morphological phenotypes of bmp4 morphants at 2 dpf (compare O with N) showing a C2 dorsalized phenotype (Mullins et al., 1996) involving loss of the ventral tail fin and ventral vein. Expression of a Bmp4 target gene, flk1 24 hpf, is down-regulated in bmp4 morphants (compare Q with P). Both phenotypes are observed in a previously published bmp4 null mutant (Stickney et al., 2007). Arrows denote the extent of ventral tissue loss.

Expression Data

Genes:	kdrl tbx5a
Antibody:	Ab1-smad
Fish:	WT WT + MO3-bmp4 + MO4-tp53
Knockdown Reagents:	MO3-bmp4 MO4-tp53
Anatomical Terms:	optic cup optic cup dorsal region optic vesicle post-vent region
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 333(1), French, C.R., Erickson, T., French, D.V., Pilgrim, D.B., and Waskiewicz, A.J., Gdf6a is required for the initiation of dorsal-ventral retinal patterning and lens development, 37-47, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.