Fig. 8
Lens-specific gene expression is deregulated in gdf6a morphants. The expression of lim2.3 is down-regulated at 28 hpf in gdf6a morphant embryos (A, B), as is the expression of cx23 (C, D) and crystallin ba2a (E, D). The expression of the related crystallin ba1b is also down-regulated (G, H). At 4 dpf, there is little difference in the organization of F-actin in gdf6a morphants, as no changes in phalloidin staining are observed (I, J). There is also no change in nuclear staining, indicating no residual nuclear retention in gdf6a morphants (Q, R). There is however a slightly higher level of Zl-1 staining in the morphant lens, which identifies differentiating lens fibers (M, N). Incubation in Dorsomorphin has no effect of lens development (K, O, S, W), but when combined with gdf6a morpholino inhibition, ectopic phalloidin and Zl-1 is observed in the lens (L, P), and there is evidence of nuclear retention (T). A merged image of all staining is shown in panels U–X. |
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| Stage Range: | Prim-5 to Day 4 |
Reprinted from Developmental Biology, 333(1), French, C.R., Erickson, T., French, D.V., Pilgrim, D.B., and Waskiewicz, A.J., Gdf6a is required for the initiation of dorsal-ventral retinal patterning and lens development, 37-47, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.