FIGURE

Fig. S11

ID

ZDB-FIG-090306-38

Publication

Löhr et al., 2009 - Zebrafish diencephalic A11-related dopaminergic neurons share a conserved transcriptional network with neuroendocrine cell lineages

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

Supernumerary DA neurons in Otp/Sim1OE embryos express both otp and sim1. (A-E″) Expression of sim1b (whole-mount in situ hybridization, green), otpb (whole-mount in situ hybridization, red) and TH (immunostaining, white) at 25 hpf in wild-type control (A-B′) and transgenic embryos expressing otpa from the hsp70 promoter co-injected with sim1a mRNA (Otp/Sim1OE; C-E″). Lateral view of the region of DA specification in the posterior tuberculum/hypothalamus in wild-type embryos showing co-expression of TH with sim1b (A-A″) as well as with otpb (B-B′). Lateral view of the same brain region in an Otp/Sim1OE embryo showing supernumerary ectopic TH cells (total of 16 cells) that are arranged in a rostrocaudally elongated expression domain but still express sim1b (C-C″) and otpb (D-D′), reflecting the expanded sim1b/otpb expression domains. (E-E″) Dorsal view of TH cells in an Otp/Sim1OE embryo showing that ectopic, anteriorly located TH/sim1b-positive cells send projections caudally where they join the regular DA axonal tracts (arrowheads in E,E″). Anterior is towards the left. Scale bars: 50 μm. Fluorescent images in represent z-projections from single focal planes: (A-D′) 20 μm; (E-E″) 40 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development