FIGURE

Fig. 1

ID

ZDB-FIG-090304-56

Publication

Feijoo et al., 2009 - Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a

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Fig. 1

irx4a morphant embryos show defects in head size and extensive cell death. (A, B) Images of live wild type (A) and morphant (B) embryos at 24 hpf; Compare the size of the eyes and head (arrows). (C, D) Acridine orange staining of wild type (C) and irx4a morphant (D) embryos viewed at 24 hpf. Note the increased number of labeled cells in the morphant embryo (E, F) MO4as injected embryos do not show substantial changes in cell proliferation as evidenced by anti-phospho-histone H3 (H3-P) immunostaining. (G) RT-PCR showing the effect of the MO4as on irx4a transcript splicing. RNA prepared from uninjected embryos (“C”) shows the internal control band (actin, act) but the absence of unprocessed irx4a mRNA. The presence of this band in MO4as injected fish (arrow) indicates defective irx4a mRNA splicing. Panels A–F show lateral views of 24 hpf embryos; dorsal is up, anterior is left. Scale bar in F: 70 μm.

Expression Data

Gene:	irx4a
Antibody:	Ab6-h3
Fish:	WT + MO5-irx4a
Knockdown Reagent:	MO5-irx4a
Anatomical Term:	head
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-irx4a
Knockdown Reagent:	MO5-irx4a
Observed In:	brain eye
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 40(3), Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L., Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a, 328-337, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell Neurosci.