PUBLICATION

Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a

Authors
Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L.
ID
ZDB-PUB-090106-31
Date
2009
Source
Molecular and cellular neurosciences   40(3): 328-337 (Journal)
Registered Authors
Allende, Miguel L., Feijoo, Carmen G., Gómez-Skarmeta, José Luis
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning
  • Cell Death/physiology
  • Gene Expression Regulation, Developmental
  • Neurogenesis/physiology*
  • Rhombencephalon/cytology
  • Rhombencephalon/physiology
  • SOXB1 Transcription Factors/genetics
  • SOXB1 Transcription Factors/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Trigeminal Ganglion/cytology
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 17)
PubMed
19111617 Full text @ Mol. Cell Neurosci.
Abstract
Members of the Iroquois (Irx) homeodomain transcription factor gene family have been implicated in a variety of early developmental processes, including neural pre-patterning, tissue differentiation, neural crest development and cranial placode formation. Here, we report that, in zebrafish, the irx4a gene participates in specification of a number of placode derivatives that arise from the posterior placodal field. Specifically, differentiation of the trigeminal, epibranchial and lateral line placodes are affected when irx4a function is interrupted using antisense morpholino oligonucleotides. We show that both in the trigeminal ganglion and in the lateral line, irx4a is involved in controlling the number of sensory cells that develop. Other phenotypes observed in morphant embryos include misspecification of the heart chambers and failure of retinal ganglion and photoreceptor cell differentiation, functions described previously for Irx4 in other species. We also provide evidence that irx4a regulates the expression of the sox2 gene, both in the neural plate and in progenitor cells of the lateral line system. Our results point to irx4a as a critical gene for numerous developmental processes and highlight its role in the formation of placodal derivatives in vertebrates.
Genes / Markers
Figures
Figure Gallery (10 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO5-irx4astandard conditionsPrim-5
WT + MO5-irx4astandard conditionsPrim-5
WT + MO5-irx4astandard conditionsPrim-5
WT + MO5-irx4astandard conditionsPrim-5
WT + MO5-irx4astandard conditionsPrim-5
WT + MO5-irx4astandard conditionsLong-pec
WT + MO5-irx4astandard conditionsDay 5
1 - 7 of 7
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sqet4EtTransgenic Insertion
    sqet20EtTransgenic Insertion
      zf106TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        Sequence Targeting Reagents
        Fish
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L. (2009) Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a. Molecular and cellular neurosciences. 40(3):328-337.