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Fig. S2

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ZDB-FIG-090127-30

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Suster et al., 2009 - A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals

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Fig. S2

Transient expression of pufferfish evx1:GFP reporter constructs in zebrafish embryos. All reporter constructs contained a 7 kb promoter and GFP fused in frame in exon 2 as described in Fig. 2A. Constructs with or without the 3′ evx1 region (- 3′ evx1 and + 3′ evx1) were injected into the cytoplasm of one-cell stage embryos. Fluorescence images of whole-mount living embryos were taken under a dissection microscope at ∼ 32 hpf. The region of the ‘spinal cord’ examined in our study is labeled in A and the dorso-ventral limits of the spinal cord marked by dash lines. Constructs 7 k (7 kb promoter alone), 10k, 5035F, 2933F and 1917F are described in Fig. 2A. Dorsal is up and rostral is to the left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(2), Suster, M.L., Kania, A., Liao, M., Asakawa, K., Charron, F., Kawakami, K., and Drapeau, P., A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals, 422-433, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.