|
Inhibition of p53 activity and suppression of apoptosis by p53 MO. Zebrafish embryos at the one-cell stage were injected with p53 MO and p53 misMO at 50 pg/embryo. At 24 hpf, the embryos were exposed to 400 mJ/cm UV light for 5 s. Six hours later, they were fixed for TUNEL staining and RT-PCR. (A) Semiquantitative RT-PCR of p53-target genes in UV-exposed embryos injected with p53 MO (50 pg/embryo) or p53 misMO (50 pg/embryo). The p53 response genes are completely downregulated in p53 MO-injected, but not in p53 misMO-injected, embryos. (B–E) TUNEL staining of p53 MO- and p53 misMO-injected embryos before and after UV exposure. The embryos injected with p53 MO show a complete inhibition of apoptosis (indicated by an absence of TUNEL-positive cells) after UV exposure, whereas p53 misMO-injected embryos show extensive apoptosis. All images are in lateral view with anterior to the left. Scale bar: 500 μm.
|
