FIGURE

Fig. 7

ID

ZDB-FIG-080925-7

Publication

Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient

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Fig. 7

Disruption of the Nuclear Anchor to Dynactin Phenocopies the mok^s309 Mutation

(A and B) Expression of the Müller glia marker GS is reduced in the retina of larvae injected with a syne2a-MO (B) compared to control MO-injected larvae (A). Scale bars, 100 μm.

(C and D) Representative examples of sections of 5 dpf retinas overexpressing a control vector (C) or a dominant-negative Syne2a (KASH, [D]) under the control of a heat-shock promoter. The cells that express the constructs are identified by the expression of GFP marker (green). Clones of cells that express the syne2a dominant-negative construct preferentially generate GCL neurons. Scale bars, 50 μm.

(E) Quantification of the KASH overexpression results, showing the distribution of clones in the three retinal nuclear layers. ^*p < 0.01, ^**p < 0.005. Error bar indicates SEM.

(F) A model of the mechanism that couples INM with graded Notch activation.

Expression Data

Antibody:	Ab1-glul
Fish:	TL TL + MO1-syne2a
Knockdown Reagent:	MO1-syne2a
Anatomical Term:	Muller cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-syne2a
Knockdown Reagent:	MO1-syne2a
Observed In:	Muller cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell