FIGURE

Fig. 6

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ZDB-FIG-080925-6

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Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient

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Fig. 6

Atoh7 Loss of Function and Notch Activation Can Rescue Late Cell Fates in mok^s309

(A–D) Compared to wild-type (A), mok mutants (B) have an excess of RGCs and few bipolar cells, labeled by PKCα immunoreactivity. lak (atoh7) mutants fail to generate RGCs and overproduce bipolar neurons (C). lak/mok double mutants have rescued bipolar cell production.

(E–H) Heat-shocked induction of an activated form of Notch receptor NICD at 32 hpf drives progenitors toward a Müller glia fate (GS positive) in both wild-type (G) and mok^s309 retinas (H). The presence of the transgenes (hsp:Gal4, UAS:NICD) has no effect on Müller glia differentiation in the absence of heat shock (E and F).

Scale bars, 100 μm.

Expression Data

Antibodies:	Ab1-glul Ab1-prkca
Fish:	TL atoh7^th241/th241 (TL) dctn1a^s309/s309 (TL) kca3Tg; kca4Tg atoh7^th241/th241; dctn1a^s309/s309 (TL) dctn1a^s309/s309; kca3Tg; kca4Tg (TL)
Condition:	heat shock
Anatomical Terms:	Muller cell retina
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atoh7^th241/th241 (TL) dctn1a^s309/s309 (TL) atoh7^th241/th241; dctn1a^s309/s309 (TL) dctn1a^s309/s309; kca3Tg; kca4Tg (TL)
Condition:	heat shock
Observed In:	Muller cell retinal bipolar neuron retinal ganglion cell retinal photoreceptor layer photoreceptor cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell