FIGURE

Fig. 1

ID

ZDB-FIG-080925-1

Publication

Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient

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Fig. 1

mok^s309 Mutants Have a Complex Retina Phenotype

(A and B) Whole-mount lateral views of live 4.5 dpf zebrafish larvae. In mok^s309 mutants (B), the eyes are smaller than in the wild-type (A), but external morphology is otherwise indistinguishable.

(C and D) Horizontal sections of 5 dpf retinas stained with DAPI (blue) and Zpr1 antibody (green). mok^s309 retinas (D) have an expanded GCL (dashed white lines), compared to wild-type (C), and no photoreceptors, as revealed by Zpr1 staining.

(E and F) Coronal sections of 3 dpf retinas stained by TUNEL assay. mok^s309 retinas (F) have increased apoptosis, particularly in the photoreceptor layer (asterisks), compared to wild-type (E).

(G and H) Horizontal sections of 48 hpf retinas expressing GFP driven by the atoh7 promoter (green) and stained with DAPI (blue) and HuC/D antibody (red). mok^s309 retinas (H) have an increased number of GFP-expressing RGCs compared to wild-type (G).

Scale bars, 500 μm (A and B) and 100 μm (C–H).

Expression Data

Gene:	GFP
Antibodies:	Ab1-elavl zpr-1
Fish:	TL dctn1a^s309/s309 (TL) rw021Tg rw021Tg (TL) dctn1a^s309/s309; rw021Tg (TL)
Anatomical Terms:	retinal ganglion cell layer retinal ganglion cell retinal photoreceptor layer photoreceptor cell
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dctn1a^s309/s309 (TL) dctn1a^s309/s309; rw021Tg (TL)
Observed In:	eye lens retinal ganglion cell layer retinal ganglion cell retinal photoreceptor layer photoreceptor cell
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell