FIGURE

Fig. 6

ID

ZDB-FIG-080402-22

Publication

Christensen et al., 2008 - Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development

Other Figures

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Fig. 6

The outer limiting membrane (OLM) is not restored in moe^-mutants by injection of epb4.1l5^longmRNA. (A) In wild-type larvae at 6 dpf, Crumbs proteins localize just apical to the OLM, which is labeled by anti-ZO-1. (B) In moe^-mutants, very little Crumbs protein is visible and ZO-1 labeling is disorganized. (C) In epb4.1l5^longmRNA injected moe^-mutants, streaks of panCrb labeling are visible, but an organized OLM is absent. Ultrastructural transmission electron microscopic analysis at 6 dpf in wild-type (D), moe^-mutants (E) and epb4.1l5^longmRNA injected moe^-mutants (F) retinas. Electron dense outer segments are seen in all individuals. Insets, higher magnifications of rod outer segments showing regular disc stacking is present in all individuals (100,000X). RPE, retinal pigmented epithelium; OS, outer segments; IS, inner Segments; CB, cell body. (A-C) are confocal z-projections, scale bar 10 μm (D-F). Scale bars, 5 μm (D-F), 100 nm (insets).

Expression Data

Genes:	EGFP tjp1a
Fish:	fl1Tg epb41l5^b781/b781; fl1Tg
Anatomical Terms:	outer limiting membrane retina retinal rod cell
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	epb41l5^b781/b781; fl1Tg
Observed In:	outer limiting membrane
Stage:	Day 6

Phenotype Detail

Acknowledgments

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