FIGURE

Fig. 7

ID
ZDB-FIG-080325-26
Publication
Hendricks et al., 2008 - Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation
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Fig. 7

wnt4a and dnRyk affect commissure formation. Lateral (A) and dorsal (B) view of a 48 hpf embryo, showing expression of wnt4a in the medial habenula. (C) A 3 dpf embryo after injection with a morpholino (MO1) to wnt4a. Habenular afferents have entered the habenula and reached the medial edge (arrows) but have not crossed the roof plate. Axons have innervated the habenula neuropil (arrowhead), which normally occurs only after the commissure has formed. (D) An embryo at 3 dpf after unilateral electroporation with HuC:Gal4/UAS:dnRyk-EGFP at 1 dpf. Abnormal loops and branches are seen within the commissure (n = 15). (E, F) Time series of individual axons show that growth cones reach the contralateral boundary, then bifurcate (arrowheads), turn around and recross the midline (see Supplementary Movies 5 and 6). Time series in (E) was taken without a heated stage, causing slower growth. Time is h:min.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
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Reprinted from Molecular and cellular neurosciences, 37(2), Hendricks, M., Mathuru, A.S., Wang, H., Silander, O., Kee, M.Z., and Jesuthasan, S., Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation, 271-283, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.