plcβ3 is required for edn1-dependent Dlx gene expression. Lateral views of in situ hybridizations in wild-type and plcβ3 homozygotes at 30 hpf (A–H) and 36 hpf (I–P). (A, E, I, M) dlx2a, (B, F, J, N) dlx5a, (C, G, K, O) dlx6a, and (D, H, L, P) dlx3b. At 30 hpf, expression of dlx2a is moderately affected in the most ventral domain in plcβ3 homozygotes (E), and at 36 hpf dlx2a expression is clearly reduced in a ventral arch domain in both the first and second arches in plcβ3 homozygotes (M). At 30 hpf, the expressions of dlx3b, dlx5a, and dlx6a are strongly reduced in both the first and second arches in plcβ3 homozygotes (F, G, H). At 36 hpf, expressions of dlx6a and dlx3b are still strongly reduced in plcβ3 homozygotes (O, P). At 36 hpf, expression of dlx5a has recovered in a dorsal arch domain but is still strongly reduced in intermediate and ventral arch domains (N). a1 and a2 label pharyngeal arch 1 and 2. Dotted lines label pharyngeal pouches 1 and 2 in panels A–P and bottom of arch 1 and 2 in panels F, N, G, O, H, and P. Scale bar: 50 μm.
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