Fig. 3

A splice-blocking MO partially rescues plcβ3 homozygotes. (A–D) Flatmounts of 5–6 dpf wild-type and plcβ3 homozygotes pharyngeal tissues doubly stained with Alcian blue and Alizarin red for cartilage and bone, respectively. (A) Uninjected wild type. (B) Injection of plcβ3 splice-blocking MO into wild-type yields only mild phenotypes. Arrow indicates reduction of symplectic cartilage. (C) plcβ3 homozygotes have a loss of jaw joints, misshaped palatoquadrate cartilage, and a strong reduction of symplectic (arrows) and ceratohyal cartilages (arrowheads). (D) Injection of plcβ3 splice-blocking MO into plcβ3 heterozygotes partially rescues cartilage defects. Arrows indicate reduction of symplectic cartilage. Asterisks indicate ectopic cartilage nodule extending from palatoquadrate. Jaw joints and ceratohyal cartilages are strongly rescued. Scale bar: 50 μm.