FIGURE

Fig. 2

ID

ZDB-FIG-070914-42

Publication

McCollum et al., 2007 - A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3

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Fig. 2

Loss of lmo4b causes forebrain and eye enlargement. (A, B) Lateral view of 10-somite embryos injected with 4 ng of 4 mm-MO (A) and 4 ng of lmo4b-MO (B). (C) Quantitative representation of eye diameter (ED) and head height (HH) measurements of embryos injected with 4 mm-MO (blue) and lmo4b-MO (red). Error bars represent standard error of the mean (S.E.M.). Values in μm with confidence intervals for head height are 131 ± 8 for control injections and 162 ± 12 for morphants, and for eye diameter are 125 ± 10 for control injections and 164 ± 12 for morphants. For HH p = 8.7 x 10^- ⁵, for ED p = 2.2 x 10^- ⁵. (D–O) Whole mount views of embryos injected with 4 mm-MO (D, F, H, J, L, N) and lmo4b-MO (E, G, I, K, M, O) show size differences in the forebrain and eyes. Panels D, E, and J–M are lateral views. Panels F–I are dorsal views of the anterior region, and panels N, O are ventral views of the anterior. The dashed lines in panels L, M represent pre-optic area. tel = telencephalon; di = diencephalon. Scale bars are 20 μm.

Expression Data

Genes:	nkx2.4b pax2a pax6b zic1
Fish:	WT WT + MO2-lmo4a + MO3-lmo4a
Knockdown Reagents:	MO2-lmo4a MO3-lmo4a
Anatomical Terms:	diencephalon hypothalamus optic stalk optic vesicle telencephalon
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-lmo4a + MO3-lmo4a
Knockdown Reagents:	MO2-lmo4a MO3-lmo4a
Observed In:	diencephalon eye head hypothalamus optic stalk optic vesicle telencephalon
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 309(2), McCollum, C.W., Amin, S.R., Pauerstein, P., and Lane, M.E., A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3, 373-385, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.