Fig. 1
- ID
- ZDB-FIG-070914-40
- Publication
- McCollum et al., 2007 - A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3
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Zebrafish lmo4b is essential for viability and eye, ear and pectoral fin development. (A) Alignment of amino acid sequence alignment of zebrafish (Dr.) Lmo4b (Accession #NP817093) with mouse (Mm.; Accession #NM010723.1), human (Hs.; Accession #NM006769.2) and zebrafish (Dr.) Lmo4a (Accession #AAN03596). Residues unique to Lmo4b are underlined in red. (B) Western blot of extracts from embryos injected with 500 pg of 6xmyc-lmo4b mRNA and a mixture of two non-overlapping translational-blocking MOs or a 4 mm control MO, probed with anti-myc antibody. An unrelated immunoreactive band present in all extracts demonstrates comparable loading for all lanes. (C–J) 72 hpf larvae or larval tissue from embryos injected with 4 ng of either 4 mm-MO (C, E, G, I) or 4 ng of lmo4b-MO (D, F, H, J). High magnification views of morphant eyes reveal retinal enlargement (F) and defects in the ear (G, H) and pectoral fins (I, J). |
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| Stage: | Protruding-mouth |
Reprinted from Developmental Biology, 309(2), McCollum, C.W., Amin, S.R., Pauerstein, P., and Lane, M.E., A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3, 373-385, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.