FIGURE

Fig. 6

ID

ZDB-FIG-070822-60

Publication

Kloosterman et al., 2007 - Targeted Inhibition of miRNA Maturation with Morpholinos Reveals a Role for miR-375 in Pancreatic Islet Development

Other Figures

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Fig. 6

Specific Effects of miR-375 Knockdown on the Development of the Endocrine Pancreas (A) In situ analysis of miR-375 and insulin expression in 72-hpf embryos injected with morpholinos against the miR-375 precursor and negative control morpholinos for let-7 and miR-124. (B) Expression of islet1, foxa2, and ptf1a in wild-type and miR-375 knockdown embryos. Arrows indicate the pancreatic islet. WT, wild type

Expression Data

Genes:	dre-mir-375-1 dre-mir-375-2 foxa2 ins isl1a ptf1a
Fish:	WT WT + MO1-dre-mir-375-1 WT + MO1-dre-mir-375-2 WT + MO2-dre-mir-375-1 WT + MO2-dre-mir-375-2 WT + MO1-dre-mir-375-1 + MO1-dre-mir-375-2 WT + MO1-dre-mir-375-1,dre-mir-375-2 WT + MO2-dre-mir-375-1 + MO2-dre-mir-375-2 WT + MO2-dre-mir-375-1,dre-mir-375-2
Knockdown Reagents:	MO1-dre-mir-375-1 MO1-dre-mir-375-1,dre-mir-375-2 MO1-dre-mir-375-2 MO2-dre-mir-375-1 MO2-dre-mir-375-1,dre-mir-375-2 MO2-dre-mir-375-2
Anatomical Terms:	endocrine pancreas exocrine pancreas gut liver pancreatic bud
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dre-mir-375-1 WT + MO1-dre-mir-375-2 WT + MO2-dre-mir-375-1 WT + MO2-dre-mir-375-2 WT + MO1-dre-mir-375-1 + MO1-dre-mir-375-2 WT + MO1-dre-mir-375-1,dre-mir-375-2 WT + MO2-dre-mir-375-1 + MO2-dre-mir-375-2 WT + MO2-dre-mir-375-1,dre-mir-375-2
Knockdown Reagents:	MO1-dre-mir-375-1 MO1-dre-mir-375-1,dre-mir-375-2 MO1-dre-mir-375-2 MO2-dre-mir-375-1 MO2-dre-mir-375-1,dre-mir-375-2 MO2-dre-mir-375-2
Observed In:	endocrine pancreas exocrine pancreas
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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