FIGURE

Fig. 5

ID

ZDB-FIG-070822-59

Publication

Kloosterman et al., 2007 - Targeted Inhibition of miRNA Maturation with Morpholinos Reveals a Role for miR-375 in Pancreatic Islet Development

Other Figures

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Fig. 5

Knockdown of miR-375 Results in Aberrant Migration of Pancreatic Islet Cells (A) In situ analysis of miR-375 knockdown in MO miR-375–injected embryos and noninjected controls at 24, 48, 72, and 120 hpf. Arrowheads indicate the pituitary gland and the pancreatic islet. (B) In situ analysis of the pancreatic islet (insulin staining) and the pituitary gland (pit1 staining) in miR-375 morphants and noninjected controls. Arrowheads indicate the pituitary gland and the pancreatic islet. (C) In situ analysis of pancreatic islet development in wild-type and morphant embryos using insulin, somatostatin, and glucagon as markers. (D) Time series of insulin expression in wild-type and morphant embryos injected with MO miR-375. (E) Insulin expression in 72-hpf embryos injected with MO miR-375 and a complementary morpholino.

Expression Data

Genes:	dre-mir-375-1 dre-mir-375-2 gcga ins pou1f1 sst2
Fish:	WT WT + MO1-dre-mir-375-1,dre-mir-375-2
Knockdown Reagent:	MO1-dre-mir-375-1,dre-mir-375-2
Anatomical Terms:	endocrine pancreas endoderm hypophysis pancreatic bud
Stage Range:	14-19 somites to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dre-mir-375-1,dre-mir-375-2
Knockdown Reagent:	MO1-dre-mir-375-1,dre-mir-375-2
Observed In:	endocrine pancreas
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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