FIGURE

Fig. S3

ID

ZDB-FIG-051221-2

Publication

Chen et al., 2005 - Loss of function of def selectively up-regulates {Delta}113p53 expression to arrest expansion growth of digestive organs in zebrafish

Other Figures

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Fig. S3

def-MO caused hypoplastic digestive organs as that observed in the def^hi429 mutant. (A,B) def-MO (5′-ATGAATATAATGACTTACCAAGCGC-3′) was designed to target the splice junction between exon 2 and intron 2. RT-PCR analysis showed that def-MO created an aberrant frame-shift transcript (A) due to 40 bp deletion in the exon 2 (B). (MO) def-MO; (SC) standard control morpholinos; (C) wild type contol. (C) def-MO morphants were examined with intestinal marker ifabp, liver marker lfabp and pancreas marker trypsin, respectively. 19/27 (70.4%) morphants showed hypoplastic intestine tube (ifabp); 35/47 (74.5%) for liver (lfabp), and 50/56 (89.3%) for pancreas (trypsin).

Expression Data

Genes:	fabp2 fabp10a prss1
Fish:	WT WT + MO1-utp25
Knockdown Reagent:	MO1-utp25
Anatomical Terms:	gut liver pancreas
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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