Fig. 5

Expression of cyp26a1 and raldh2 is affected in the gir mutant. Expression of cyp26a1 (A–D and G–I), cyp26a1 and krox20 (E and F), and raldh2 (J–O) in the wild type (A, C, E, G, H, J, L, and N) and gir mutant (B, D, F, I, K, M, and O) embryos. (A and B) cyp26a1 expression in the presumptive anterior neural ectoderm and in the tailbud is little affected at bud stage in the gir mutant. Dorsal views are shown. (C and D) At the 5-somite stage, cyp26a1 expression in the rostral presumptive spinal cord is upregulated in the gir mutant. (E and F) At 20 hpf, cyp26a1 expression is strongly upregulated in the rostral spinal cord and in the tailbud of the gir mutant. (G) A transverse section at the level of the rostral spinal cord showing cyp26a1 expression in the wild-type embryo at 20 hpf. N, notochord. (H and I) cyp26a1 expression in the developing branchial arches (indicated by the arrowheads) is perturbed at 28 hpf in the gir mutant. (J–M) raldh2 expression in the presomitic mesoderm and in the somites is reduced in the gir mutant at bud stage (K) and 20 hpf (M). (N and O) At 28 hpf, raldh2 expression in the posterior branchial arch primordium (ba) and in the posterior fin bud- and lateral plate-mesoderm (lp) is disturbed in the gir mutant. Genotypes were determined by PCR assay after the photographs had been taken in A–F and J–M.