CRISPR

CRISPR1-sirt1

ID

ZDB-CRISPR-201105-1

Name

CRISPR1-sirt1

Previous Names

None

Target

sirt1 (1)

Sequence

5' - ACGAGAAACCGGCGCGGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
yu28	sirt1

Expression

Gene expression in Wild Types + CRISPR1-sirt1

No data available

Phenotype

Phenotype resulting from CRISPR1-sirt1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sirt1

Phenotype	Fish	Conditions	Figures
intestine atrophied, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
whole organism socs1a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
whole organism nfe2l2a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
inflammatory response increased process quality, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
reactive oxygen species biosynthetic process occurrence, ameliorated	sirt1^yu28/yu28 (AB)	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: tert-butyl hydroperoxide	Fig. 2 from Kim et al., 2019
reactive oxygen species biosynthetic process increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	control	Fig. 2 from Kim et al., 2019
reactive oxygen species biosynthetic process increased occurrence, exacerbated	sirt1^yu28/yu28 (AB)	chemical treatment by environment: tert-butyl hydroperoxide	Fig. 2 from Kim et al., 2019
whole organism decreased life span, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 5 from Kim et al., 2019
apoptotic process increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	control	Fig. 2 from Kim et al., 2019
whole organism casp3a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
whole organism sirt1 expression absent, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 1 from Kim et al., 2019
pancreas inflammatory response increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
spleen inflammatory response increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
whole organism il6 expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
apoptotic process increased occurrence, exacerbated	sirt1^yu28/yu28 (AB)	chemical treatment by environment: tert-butyl hydroperoxide	Fig. 2 from Kim et al., 2019
whole organism viability, ameliorated	sirt1^yu28/yu28 (AB)	chemical treatment by environment: tert-butyl hydroperoxide	Fig. 2 from Kim et al., 2019
whole organism socs3a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
intestine tnfa expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 5 from Kim et al., 2019
apoptotic process occurrence, ameliorated	sirt1^yu28/yu28 (AB)	chemical treatment by environment: N-acetyl-L-cysteine, chemical treatment by environment: tert-butyl hydroperoxide	Fig. 2 from Kim et al., 2019
intestine apoptotic process increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 5 from Kim et al., 2019
whole organism tnfa expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
intestine inflamed, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
intestine rela expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 5 from Kim et al., 2019
pancreas inflamed, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
whole organism tgfb1a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
spleen inflamed, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 from Kim et al., 2019
intestine mpx expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 5 from Kim et al., 2019
intestine inflammatory response increased occurrence, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 4 , Fig. 5 from Kim et al., 2019
whole organism il1b expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019
whole organism nos2a expression increased amount, abnormal	sirt1^yu28/yu28 (AB)	standard conditions	Fig. 3 from Kim et al., 2019

Citations