Effect of E₂ on 5-HT-positive area and relative expression of tph isoforms. (A) Representative images of ventral view of head region showing 5-HT-positive cells in pretectal and thalamic complex (ptc) and raphe (r) at 2 dpf. Scale bar: 200 µm. (B,C) Measurements of the area of 5-HT-positive neurons in the experiment of E₂ exposure and co-incubation of E₂ and ICI, respectively. (D,E) Semi-quantitative PCR for expression of tph isoforms in 4 dpf larvae in the experiments of exposure to low and high doses of E₂ and co-incubation with ICI, respectively. Data are presented as a mean ± SEM. Different letters in each graph indicate significant differences (p < 0.05).

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.