FIGURE SUMMARY
Title

The construction of intrahepatic cholangiocarcinoma model in zebrafish

Authors
Wang, J., Leng, X., Wang, G., Wan, X., Cao, H.
Source
Full text @ Sci. Rep.
Fig. 1

The liver tumor in Tg(fabp10:nras 61K) transgenic zebrafish. The fluorescence of EGFP was used to detect the nras 61K gene expression. (A,C,E,G) Brightfield and fluorescence (insets) images displaying the gross morphology of F4 Tg(fabp10:nras 61K) transgenic zebrafish at 3, 6, 9 and 12 mpf. Tumor protrusions are indicated by arrowheads. (B,D,F,H) Histology of F4 Tg(fabp10:nras 61K) transgenic zebrafish at 3, 6, 9 and 12 mpf. (I) Brightfield images displaying the gross morphology of WT zebrafish at 12 mpf. (J) Histology of WT zebrafish at 12 mpf. Dotted areas indicate liver regions. Scale bars: 50 µm.
Fig. 2

Histological and immunohistochemical examination of ICC in WT and Tg(fabp10:nras 61K) transgenic zebrafish (12 mpf) by hematoxylin and eosin (H&E) stain. Histological images of WT (A) and Tg(fabp10:nras 61K) transgenic zebrafish (B) at 12 mpf. (C) Histological analysis confirmed that the tumor was ICC. (D) Infiltration of cancer cell into the surrounding liver cell in the Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. CC: cancer cell; FS: focally squamoid; IC: infiltrative type cell; NC: normal cell; PP: pleomorphic plump tumor cells; Scale bars: 50 µm.
Fig. 3

Immunohistochemical examination of ICC in WT and Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Immunohistochemical images of PCNA (A), CK19 (C), phosphorylated ERK (pERK) (E), phosphorylated MEK1/2 (pMEK1/2) (G) in WT and Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Tumor surrounding tissue of Tg(fabp10:nras 61K) transgenic zebrafish liver showing intense PCNA (B), CK19 (D), pERK (F), pMEK1/2 (H) immunostaining in Tg(fabp10:nras 61K) transgenic zebrafish at 12 mpf. Immunostaining signals in the bile duct of Tg(fabp10:nras 61K) transgenic zebrafish liver are indicated by arrowheads. Scale bars: 50 µm.
Fig. S3

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EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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