PUBLICATION

Synaptically silent sensory hair cells in zebrafish are recruited after damage

Authors
Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S.
ID
ZDB-PUB-180418-24
Date
2018
Source
Nature communications   9: 1388 (Journal)
Registered Authors
Beirl, Alisha, Kindt, Katie, Li, Suna, Wong, Hiu-Tung Candy, Zhang, Qiuxiang
Keywords
none
MeSH Terms
  • Animals
  • Calcium/metabolism
  • Calcium Channels, L-Type/genetics
  • Calcium Channels, L-Type/metabolism*
  • Cations, Divalent
  • Embryo, Nonmammalian
  • Hair Cells, Auditory/cytology*
  • Hair Cells, Auditory/metabolism
  • Ion Transport
  • Larva/cytology
  • Larva/metabolism
  • Lateral Line System/growth & development
  • Lateral Line System/injuries
  • Lateral Line System/metabolism
  • Mechanotransduction, Cellular/physiology*
  • Potassium/metabolism
  • Regeneration/physiology
  • Synapses/metabolism*
  • Synaptic Transmission/physiology*
  • Zebrafish
(all 20)
PubMed
29643351 Full text @ Nat. Commun.
Abstract
Analysis of mechanotransduction among ensembles of sensory hair cells in vivo is challenging in many species. To overcome this challenge, we used optical indicators to investigate mechanotransduction among collections of hair cells in intact zebrafish. Our imaging reveals a previously undiscovered disconnect between hair-cell mechanosensation and synaptic transmission. We show that saturating mechanical stimuli able to open mechanically gated channels are unexpectedly insufficient to evoke vesicle fusion in the majority of hair cells. Although synaptically silent, latent hair cells can be rapidly recruited after damage, demonstrating that they are synaptically competent. Therefore synaptically silent hair cells may be an important reserve that acts to maintain sensory function. Our results demonstrate a previously unidentified level of complexity in sculpting sensory transmission from the periphery.
Genes / Markers
Marker Marker Type Name
ctbp2lGENEC-terminal binding protein 2, like
en.sillENHANCERcntnap2a intronic enhancer/Sensory Innervation of the Lateral Line
hsp70lGENEheat shock cognate 70-kd protein, like
neurog1GENEneurogenin 1
1 - 4 of 4
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Figures
Figure Gallery (14 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
idc1TgTransgenic Insertion
    idc3TgTransgenic Insertion
      idc6TgTransgenic Insertion
        idc7TgTransgenic Insertion
          idc8TgTransgenic Insertion
            idc9TgTransgenic Insertion
              nl1TgTransgenic Insertion
                vo10TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  neurog1MO9-neurog1MRPHLNO
                  1 - 1 of 1
                  Show
                  Fish
                  No data available
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-cacna1dapolyclonalRabbit
                  Ab1-chatpolyclonalGoat
                  Ab1-ctbp2lpolyclonalRabbit
                  Ab1-pan-MAGUKmonoclonal
                    		IgG1Mouse
                    Ab1-tubamonoclonal
                      		IgG2bMouse
                      Ab2-vamp2polyclonal
                        		IgGRabbit
                        Ab3-ctbp2lmonoclonalMouse
                        Ab5-thmonoclonalIgG2aMouse
                        1 - 8 of 8
                        Show
                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        BongwooriEFGBongwoori
                        EGFPEFGEGFP
                        GCaMPEFGGCaMP
                        mCherryEFGmCherry
                        RCaMPEFGRCaMP
                        RGECOEFGRGECO
                        SYPHYEFGSYPHY
                        1 - 7 of 7
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                        Mapping
                        No data available
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                        Citation
                        Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S. (2018) Synaptically silent sensory hair cells in zebrafish are recruited after damage. Nature communications. 9:1388.