PUBLICATION

Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish

Authors
Puttonen, H.A.J., Semenova, S., Sundvik, M., Panula, P.
ID
ZDB-PUB-170610-10
Date
2017
Source
Scientific Reports   7: 3060 (Journal)
Registered Authors
Panula, Pertti, Puttonen, Henri, Semenova, Svetlana, Sundvik, Maria
Keywords
Cellular neuroscience, Diseases of the nervous system
MeSH Terms
  • Animals
  • Antipsychotic Agents/pharmacology
  • Brain/cytology
  • Brain/metabolism
  • Brain/physiology
  • Histamine/metabolism*
  • Neurons/drug effects
  • Neurons/metabolism
  • Reserpine/pharmacology
  • Synaptic Transmission*
  • Vesicular Monoamine Transport Proteins/antagonists & inhibitors
  • Vesicular Monoamine Transport Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/metabolism*
(all 15)
PubMed
28596586 Full text @ Sci. Rep.
Abstract
Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and function of the histaminergic system in larval zebrafish following inhibition of VMAT2 function by reserpine. We found that reserpine treatment greatly reduced histamine immunoreactivity in neurons and an almost total disappearance of histamine-containing nerve fibers in the dorsal telencephalon and habenula, the most densely innervated targets of the hypothalamic histamine neurons. The reserpine treated larvae had an impaired histamine-dependent dark-induced flash response seen during the first second after onset of darkness, implying that function of the histaminergic network is VMAT2 dependent. Levels of histamine and other monoamines were decreased in reserpine treated animals. This study provides conclusive evidence of the relevance of VMAT2 in histaminergic neurotransmission, further implying that the storage and release mechanism of neural histamine is comparable to that of other monoamines. Our results also reveal potential new insights about the roles of monoaminergic neurotransmitters in the regulation of locomotion increase during adaptation to darkness.
Genes / Markers
Marker Marker Type Name
dbhGENEdopamine beta-hydroxylase (dopamine beta-monooxygenase)
hdcGENEhistidine decarboxylase
slc18a1GENEsolute carrier family 18 member A1
slc18a2GENEsolute carrier family 18 member 2
slc6a2GENEsolute carrier family 6 member 2
slc6a3GENEsolute carrier family 6 member 3
slc6a4aGENEsolute carrier family 6 member 4a
slc6a4bGENEsolute carrier family 6 member 4b
thGENEtyrosine hydroxylase
th2GENEtyrosine hydroxylase 2
1 - 10 of 12
Show
Figures
Figure Gallery (2 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment: reserpineDay 4
1 - 1 of 1
Show
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
Show
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Puttonen, H.A.J., Semenova, S., Sundvik, M., Panula, P. (2017) Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish. Scientific Reports. 7:3060.