Fig 7

Fig 7 Analysis of blood cell type markers in 3 dpf wild-type and miR-34a-/- mutants reveals erythrocyte up-regulation in the mutants.

(A) Bar graph of normalized relative expression values of blood-related genes and tp53 in the 72 hpf RNA-seq dataset comprising data on 3 wild-type and miR-34a^-/- RNA samples. (B) qPCR verification of the selected blood cell type markers on 3dpf wild-type and miR-34a^-/- RNA samples (n = 10 and 11, respectively). Significantly different genes in (A) and (B) determined by a two-sample t-test with multiple-testing adjustment are indicated by ‘***’ (P-value < 0.001), ‘**’ (P-value < 0.01) and ‘*’ (P-value < 0.05). (C) Representative ventral images of o-dianisidine stained embryos at 3 dpf of both genotypes. The numbers of larvae are indicated. The staining was performed on embryos from two independent samples. (D) Quantification of o-dianisidine staining on 3dpf wild-type and miR-34a^-/- larvae (n = 48 and 42, respectively) using the ilastik-Cell Profiler pixel classification approach. Relative values of “Mean Intensity after thresholding” positively classified pixels are shown. The significance of the differences between the genotypes was calculated by the two-sample t-test (***; P-value < 9.956e-07).