TA in hNPCs but not in terminally differentiated neuron cells transfected with unstable hAPP695 mRNA. A, Relative APP level in hNPCs transfected with uncapped eGFP (control) or hAPP695 mRNA (N = 9). B, APLP1 and APLP2 levels in hNPCs transfected with uncapped eGFP (control) or hAPP695 mRNA (N = 9). C, Relative APP level in human neuron cells transfected with uncapped eGFP or hAPP695 mRNA (N = 9). D, Relative mRNA level of APLP1 and APLP2 in terminally differentiated neuron cells transfected with uncapped eGFP or hAPP695 mRNA (N = 13). APP in control eGFP transfected cells were set at 1. A–D, n = 3 biologically independent samples. Data are mean + SD. Student's t test was used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. ns, nonsignificant.
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