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Fig. 8 Knock-down of IDO1 abolished naringin-mediated suppression of fibrosis in zebrafish. (A) Molecular docking of Naringin and IDO1. (B) The qPCR analysis of IDO1 mRNA expression in zebrafish larvae (n = 3). (C) Sirius red staining of zebrafish larvae. Figures are magnified at ×200 (n = 8). (D) Oil red O staining of zebrafish larvae. Figures are magnified at ×100 (n = 8). (E) Frozen liver sections of zebrafish larvae with liver-specific eGFP expression were immunofluorescently stained with TGF-β and Desmin (n = 7). (F and G) The qPCR analysis of α-SMA and TNF-α mRNA expression in zebrafish larvae (n = 3). The mRNA expression was normalized to β-actin mRNA expression and presented as a fold change compared with the control group. ns denotes no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.