FIGURE 1

FIGURE 1

Generation of the zebrafish models of melanocyte regeneration and melanoma (A) Following neocuproine (NCP) treatment, caudal fins of individual zebrafish were collected for each group (control, 1 dpa = proliferative or early regeneration stage, and 7 dpa = differentiation or late regeneration stage) and used as biological replicates (no pooling). (B) The zebrafish melanoma model was generated by outcrossing the Tg(mitfa:Hsa.HRAS^G12V,mitfa:GFP) line with the wild-type (wt) AB zebrafish. 50% of the siblings (mitfa+/−) were used as the control group. 50% of the siblings Tg (mitfa+/−,HRAS^G12V:GFP) were used to collect the nevi and melanoma tissues. Caudal fins were collected for each group and used as biological replicates (no pooling). dpa: days post-ablation.