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Figure 6

Relative mRNA and protein expression levels of larvae zebrafish ER stress factors in physiological condition at 5 dpf. mRNA levels were analyzed by qPCR and protein contents by western blot in (A, C) wfs1aC825X and (B, D) wfs1bW493X. zeif2α and Stain-free™ were used as controls in qPCR and western blot analysis, respectively. The expression levels of mRNA and protein are represented as the fold change from the control group. Data are expressed in mean ± SEM, n = 5–9 in each group. *P < 0.05, ***P < 0.001; unpaired t-test.

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Acknowledgments
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