ZFIN Image: Huang et al., 2022, Figure 4

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Figure Caption

Figure 4

Expression of human NELFB in hematopoietic cells partially rescues granulopoiesis in nelfb^−/− embryos. (A) Alignment of human, mouse, and zebrafish NELFB proteins. Identical and similar amino acids are shaded in gray. (B) Generation of Tg(drl:hNELFB-2A-GFP) fish and GFP expression. (C) WISH for scl or pu.1 in WT and Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (D) Quantification of WISH results in (C) (n = 40–60 embryos per group). (E) WISH for mpx in WT, Tg(drl:hNELFB-2A-GFP), nelfb^−/− and nelfb^−/−; Tg(drl:hNELFB-2A-GFP) embryos at 22 hpf. (F) Quantification of WISH results in (E) (n = 20–30 embryos per group). All results are presented as the mean ± SD from three independent experiments (t test, * for p < 0.05). “+++” and “+” in (D,F) respectively represent strong staining and weak staining. hNELFB, human NELFB.

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Acknowledgments

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