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Figure 7
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Figure 7
Whole-mount RNA in situ hybridization of the brain region of eed+/+ and eed−/− siblings at 5 dpf: (a) whole-mount in situ hybridization to detect eed (left) and phox2bb RNA expression. The red arrow shows the phox2bb ectopic expression in the retina of eed mutants. Scale bar is 500 µm; (b) whole-mount in situ hybridization to detect the expression profiles of stemness (bmi1a, sox2), proliferation (pcna, mycn), cell cycle (ccna2, ccnd1), and neuronal/glial precursor (neurod1, mag) markers. The red arrows emphasize expression profile differences between eed+/+ and eed−/− larvae. Scale bar is 500 µm.
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