ZFIN Image: Sun et al., 2021, Figure 1

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Figure 1

Excessive exercise causes skeletal muscle atrophy in zebrafish. (A) Representative image of excessive exercise and control zebrafish. (B) Changes in the body weight of zebrafish with or without excessive exercise over a 4-week period. (C) Representative photomicrographs of muscle sections stained with H&E. Scale bars = 20 μm. (D) Average fiber size (based on H&E staining). (E) The expression of muscle-atrophy-related genes (trim63a, trim63b, fbxo25, fbxo32, capn1, and casp3a), cyclin (ccnd1, ccne1, and ccng2), mitochondrial function markers (cyc1, nd4, and nd5), and ppargc1a in muscle tissue detected using real-time PCR. (F–I) Western blot analysis of Murf and Fbox32 proteins (representative blot). All uncut western blot images are provided in Supplementary Materials. Data represent mean ± SD (n = 6). (J,K) The critical swimming speed (U_crit) and oxygen consumption (MO₂) were measured using the Loligo System. * p < 0.05, ** p < 0.01, and *** p < 0.001.

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Acknowledgments

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